Pseudomonas aeruginosa PAO1, PA3875 (narG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3875
Name
narG
Replicon chromosome
Genomic location 4338261 - 4342046 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252564.1
GI 15599070
Affymetrix PA3875_narG_at
Entrez 879780
GenBank AAG07262.1
INSDC AAG07262.1
NCBI Locus Tag PA3875
protein_id(GenBank) gb|AAG07262.1|AE004804_10|gnl|PseudoCAP|PA3875
TIGR NTL03PA03875
UniParc UPI00000D4226
UniProtKB Acc G3XCX1
UniProtKB ID G3XCX1_PSEAE
UniRef100 UniRef100_G3XCX1
UniRef50 UniRef50_P09152
UniRef90 UniRef90_A0A024HBU1

Product

Feature Type CDS
Coding Frame 1
Product Name
respiratory nitrate reductase alpha chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (140.97 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.47
Kyte-Doolittle Hydrophobicity Value -0.482
Molecular Weight (kDa) 141.0
Isoelectric Point (pI) 6.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 244 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001796 (372 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3875
Search term: narG

Human Homologs

References

Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.
Sodergren EJ, Hsu PY, DeMoss JA
J Biol Chem 1988 Nov 5;263(31):16156-62
PubMed ID: 3053688
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344