Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4003
|
Name |
pbpA
Synonym: mrdA |
Replicon | chromosome |
Genomic location | 4483396 - 4485336 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252692.1 |
GI | 15599198 |
Affymetrix | PA4003_pbpA_at |
Entrez | 878962 |
GenBank | AAG07390.1 |
INSDC | AAG07390.1 |
NCBI Locus Tag | PA4003 |
protein_id(GenBank) | gb|AAG07390.1|AE004817_14|gnl|PseudoCAP|PA4003 |
TIGR | NTL03PA04003 |
UniParc | UPI00000D4222 |
UniProtKB Acc | G3XCV7 |
UniProtKB ID | G3XCV7_PSEAE |
UniRef100 | UniRef100_G3XCV7 |
UniRef50 | UniRef50_G8PXZ8 |
UniRef90 | UniRef90_G3XCV7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
penicillin-binding protein 2
|
Synonyms | |
Evidence for Translation |
Detected using LC-MS/MS (72.21 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 5.83 |
Kyte-Doolittle Hydrophobicity Value | -0.332 |
Molecular Weight (kDa) | 72.2 |
Isoelectric Point (pI) | 8.49 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7KIS | HYDROLASE/Inhibitor/Antibiotic | 10/24/20 | Crystal structure of Pseudomonas aeruginosa PBP2 in complex with WCK 5153 | Pseudomonas aeruginosa | 2.869 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Cefepime | DB01413 | DrugBank | 5.1.4 | Q9X6V3 | Penicillin-binding protein 2 | 100.0 | 646 | 0.0e+00 |
Ceftazidime | DB00438 | DrugBank | 5.1.4 | Q9X6V3 | Penicillin-binding protein 2 | 100.0 | 646 | 0.0e+00 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 575 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001680 (534 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4003
Search term: pbpA
Search term: penicillin-binding protein 2
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli.
Tamaki S, Matsuzawa H, Matsuhashi M
J Bacteriol 1980 Jan;141(1):52-7
PubMed ID: 6243629
|
Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12.
Asoh S, Matsuzawa H, Ishino F, Strominger JL, Matsuhashi M, Ohta T
Eur J Biochem 1986 Oct 15;160(2):231-8
PubMed ID: 3533535
|
Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon.
Matsuzawa H, Asoh S, Kunai K, Muraiso K, Takasuga A, Ohta T
J Bacteriol 1989 Jan;171(1):558-60
PubMed ID: 2644207
|
Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression.
Vinella D, Joseleau-Petit D, Thévenet D, Bouloc P, D'Ari R
J Bacteriol 1993 Oct;175(20):6704-10
PubMed ID: 8407846
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|