Pseudomonas aeruginosa PAO1, PA4022 (hdhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4022
Name
hdhA
Synonym: exaC2
Replicon chromosome
Genomic location 4502271 - 4503791 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentPart of the PA4022-eat-eutBC operon responsible for ethanolamine catabolism.

Cross-References

RefSeq NP_252711.1
GI 15599217
Entrez 879017
GenBank AAG07409.1
INSDC AAG07409.1
NCBI Locus Tag PA4022
protein_id(GenBank) gb|AAG07409.1|AE004819_2|gnl|PseudoCAP|PA4022
TIGR NTL03PA04022
UniParc UPI00000C5BC7
UniProtKB Acc Q9HX05
UniProtKB ID Q9HX05_PSEAE
UniRef100 UniRef100_U9IY98
UniRef50 UniRef50_P37685
UniRef90 UniRef90_A0A024HMC9

Product

Feature Type CDS
Coding Frame 1
Product Name
hydrazone dehydrogenase, HdhA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (54.98 kDa, PMID:21751344)
Charge (pH 7) -9.55
Kyte-Doolittle Hydrophobicity Value -0.079
Molecular Weight (kDa) 55.0
Isoelectric Point (pI) 5.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 595 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001661 (779 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4022
Search term: hdhA

Human Homologs

Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 2 family member [Source:HGNC Symbol;Acc:HGNC:404]
E-value: 2.3e-97
Percent Identity: 41.3

References

aldB, an RpoS-dependent gene in Escherichia coli encoding an aldehyde dehydrogenase that is repressed by Fis and activated by Crp.
Xu J, Johnson RC
J Bacteriol 1995 Jun;177(11):3166-75
PubMed ID: 7768815
Cytochrome c550 is an essential component of the quinoprotein ethanol oxidation system in Pseudomonas aeruginosa: cloning and sequencing of the genes encoding cytochrome c550 and an adjacent acetaldehyde dehydrogenase.
Schobert M, Görisch H
Microbiology (Reading) 1999 Feb;145 ( Pt 2):471-481
PubMed ID: 10075429
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Group X aldehyde dehydrogenases of Pseudomonas aeruginosa PAO1 degrade hydrazones.
Taniyama K, Itoh H, Takuwa A, Sasaki Y, Yajima S, Toyofuku M, Nomura N, Takaya N
J Bacteriol 2012 Mar;194(6):1447-56
PubMed ID: 22267508
Ethanolamine Catabolism in Pseudomonas aeruginosa PAO1 Is Regulated by the Enhancer-Binding Protein EatR (PA4021) and the Alternative Sigma Factor RpoN.
Lundgren BR, Sarwar Z, Pinto A, Ganley JG, Nomura CT
J Bacteriol 2016 Sep 1;198(17):2318-29
PubMed ID: 27325678