Pseudomonas aeruginosa PAO1, PA4416 (murF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4416
Name
murF
Replicon chromosome
Genomic location 4949772 - 4951148 (- strand)

Cross-References

RefSeq NP_253106.1
GI 15599612
Affymetrix PA4416_murF_at
Entrez 881289
GenBank AAG07804.1
INSDC AAG07804.1
NCBI Locus Tag PA4416
protein_id(GenBank) gb|AAG07804.1|AE004856_15|gnl|PseudoCAP|PA4416
TIGR NTL03PA04417
UniParc UPI00000C5D04
UniProtKB Acc Q9HVZ7
UniProtKB ID Q9HVZ7_PSEAE
UniRef100 UniRef100_W0YYS3
UniRef50 UniRef50_Q87WY1
UniRef90 UniRef90_Q02H24

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
Product Name Confidence: Class 2
Synonyms D-ala-D-ala-adding enzyme
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.64
Kyte-Doolittle Hydrophobicity Value 0.162
Molecular Weight (kDa) 47.4
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4CVL LIGASE 03/28/14 PaMurF in complex with AMP-PNP PSEUDOMONAS AERUGINOSA PAO1 2.98 X-RAY DIFFRACTION 99.5
4CVM LIGASE 03/28/14 PaMurF in complex with AMP-PNP and UDP-MurNAc-tripeptide (mDAP) PSEUDOMONAS AERUGINOSA PAO1 2.06 X-RAY DIFFRACTION 99.5
4CVK LIGASE 03/28/14 PaMurF in complex with UDP-MurNAc-tripeptide (mDAP) PSEUDOMONAS AERUGINOSA PAO1 1.92 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 577 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004006 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4416
Search term: murF

References

Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli.
Parquet C, Flouret B, Mengin-Lecreulx D, van Heijenoort J
Nucleic Acids Res 1989 Jul 11;17(13):5379
PubMed ID: 2668880
Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli.
Duncan K, van Heijenoort J, Walsh CT
Biochemistry 1990 Mar 6;29(9):2379-86
PubMed ID: 2186811
Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion.
Anderson MS, Eveland SS, Onishi HR, Pompliano DL
Biochemistry 1996 Dec;35(50):16264-9
PubMed ID: 8973200
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS
Biochemistry 1997 May;36(20):6223-9
PubMed ID: 9166795