Pseudomonas aeruginosa PAO1, PA4521 (ampE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4521
Name
ampE
Synonym: ampE
Replicon chromosome
Genomic location 5063941 - 5064777 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253211.1
GI 15599717
Affymetrix PA4521_at
Entrez 881104
GenBank AAG07909.1
INSDC AAG07909.1
NCBI Locus Tag PA4521
protein_id(GenBank) gb|AAG07909.1|AE004866_3|gnl|PseudoCAP|PA4521
TIGR NTL03PA04522
UniParc UPI00000D427B
UniProtKB Acc G3XD69
UniProtKB ID G3XD69_PSEAE
UniRef100 UniRef100_G3XD69
UniRef50 UniRef50_Q02GS0
UniRef90 UniRef90_Q02GS0

Product

Feature Type CDS
Coding Frame 1
Product Name
AmpE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.64
Kyte-Doolittle Hydrophobicity Value 0.785
Molecular Weight (kDa) 30.8
Isoelectric Point (pI) 9.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 54 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004101 (545 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4521
Search term: ampE
Search term: AmpE

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Signalling proteins in enterobacterial AmpC beta-lactamase regulation.
Lindquist S, Galleni M, Lindberg F, Normark S
Mol Microbiol 1989 Aug;3(8):1091-102
PubMed ID: 2691840
Regulation of enterobacterial cephalosporinase production: the role of a membrane-bound sensory transducer.
Honoré N, Nicolas MH, Cole ST
Mol Microbiol 1989 Aug;3(8):1121-30
PubMed ID: 2607970
Coordinate regulation of beta-lactamase induction and peptidoglycan composition by the amp operon.
Tuomanen E, Lindquist S, Sande S, Galleni M, Light K, Gage D, Normark S
Science 1991 Jan 11;251(4990):201-4
PubMed ID: 1987637
An ampD gene in Pseudomonas aeruginosa encodes a negative regulator of AmpC beta-lactamase expression.
Langaee TY, Dargis M, Huletsky A
Antimicrob. Agents Chemother. 1998 Dec;42(12):3296-300
PubMed ID: 9835532