Pseudomonas aeruginosa PAO1, PA4526 (pilB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4526
Name
pilB
Replicon chromosome
Genomic location 5069763 - 5071463 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253216.1
GI 15599722
Affymetrix PA4526_pilB_at
Entrez 879459
GenBank AAG07914.1
INSDC AAG07914.1
NCBI Locus Tag PA4526
protein_id(GenBank) gb|AAG07914.1|AE004866_8|gnl|PseudoCAP|PA4526
TIGR NTL03PA04527
UniParc UPI0000131AC4
UniProtKB Acc P22608
UniProtKB ID PILB_PSEAE
UniRef100 UniRef100_P22608
UniRef50 UniRef50_P22608
UniRef90 UniRef90_P22608

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilB
Product Name Confidence: Class 1
Synonyms pilin biogenesis protein PilB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.47
Kyte-Doolittle Hydrophobicity Value -0.249
Molecular Weight (kDa) 62.3
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 518 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004107 (418 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4526
Search term: pilB

Human Homologs

References

Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili.
Nunn D, Bergman S, Lory S
J Bacteriol 1990 Jun;172(6):2911-9
PubMed ID: 1971619