Pseudomonas aeruginosa PAO1, PA5351 (rubA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5351
Name
rubA1
Replicon chromosome
Genomic location 6019181 - 6019348 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254038.1
GI 15600544
Affymetrix PA5351_at
DNASU PaCD00008317
Entrez 879562
GenBank AAG08736.1
INSDC AAG08736.1
NCBI Locus Tag PA5351
protein_id(GenBank) gb|AAG08736.1|AE004947_6|gnl|PseudoCAP|PA5351
TIGR NTL03PA05352
UniParc UPI00000C5FBA
UniProtKB Acc Q9HTK7
UniProtKB ID RUBR1_PSEAE
UniRef100 UniRef100_Q9HTK7
UniRef50 UniRef50_Q9AL94
UniRef90 UniRef90_Q9HTK7

Product

Feature Type CDS
Coding Frame 1
Product Name
Rubredoxin 1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.13
Kyte-Doolittle Hydrophobicity Value -0.289
Molecular Weight (kDa) 6.3
Isoelectric Point (pI) 3.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 301 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004918 (839 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5351
Search term: rubA1
Search term: Rubredoxin 1

Human Homologs

References

Rubredoxins involved in alkane oxidation.
van Beilen JB, Neuenschwander M, Smits TH, Roth C, Balada SB, Witholt B
J Bacteriol 2002 Mar;184(6):1722-32
PubMed ID: 11872724
Rubredoxin from Clostridium thermosaccharolyticum. Amino acid sequence, mass-spectrometric and preliminary crystallographic data.
Meyer J, Gagnon J, Sieker LC, Van Dorsselaer A, Moulis JM
Biochem J 1990 Nov 1;271(3):839-41
PubMed ID: 2244884
Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1.
Haspel G, Ehrt S, Hillen W
Microbiology (Reading) 1995 Jun;141 ( Pt 6):1425-1432
PubMed ID: 7670642