Pseudomonas aeruginosa PAO1, PA5421 (fdhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5421
Name
fdhA
Replicon chromosome
Genomic location 6101884 - 6103083 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254108.1
GI 15600614
Affymetrix PA5421_fdhA_at
Entrez 880053
GenBank AAG08806.1
INSDC AAG08806.1
NCBI Locus Tag PA5421
protein_id(GenBank) gb|AAG08806.1|AE004954_8|gnl|PseudoCAP|PA5421
TIGR NTL03PA05422
UniParc UPI00000C5FF8
UniProtKB Acc Q9HTE3
UniProtKB ID FADH_PSEAE
UniRef100 UniRef100_Q9HTE3
UniRef50 UniRef50_Q52078
UniRef90 UniRef90_Q9HTE3

Product

Feature Type CDS
Coding Frame 1
Product Name
glutathione-independent formaldehyde dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.85
Kyte-Doolittle Hydrophobicity Value 0.029
Molecular Weight (kDa) 42.0
Isoelectric Point (pI) 6.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4JLW OXIDOREDUCTASE 03/13/13 Crystal structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 531 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003315 (1467 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5421
Search term: fdhA

Human Homologs

Ensembl 94, assembly GRCh38.p12
alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide [Source:HGNC Symbol;Acc:HGNC:256]
E-value: 4.3e-15
Percent Identity: 29.7

References

Cloning and high-level expression of the glutathione-independent formaldehyde dehydrogenase gene from Pseudomonas putida.
Ito K, Takahashi M, Yoshimoto T, Tsuru D
J. Bacteriol. 1994 May;176(9):2483-91
PubMed ID: 8169197