Pseudomonas aeruginosa PAO1, PA0176 (aer2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0176
Name
aer2
Replicon chromosome
Genomic location 199600 - 201639 (- strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248866.1
GI 15595374
Affymetrix PA0176_at
Entrez 882219
GenBank AAG03566.1
INSDC AAG03566.1
NCBI Locus Tag PA0176
protein_id(GenBank) gb|AAG03566.1|AE004455_7|gnl|PseudoCAP|PA0176
TIGR NTL03PA00177
UniParc UPI00000C4F7E
UniProtKB Acc Q9I6V6
UniProtKB ID Q9I6V6_PSEAE
UniRef100 UniRef100_Q9I6V6
UniRef50 UniRef50_Q9I6V6
UniRef90 UniRef90_Q9I6V6

Product

Feature Type CDS
Coding Frame 1
Product Name
aerotaxis transducer Aer2
Product Name Confidence: Class 1
Synonyms methyl-accepting chemotaxis protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -18.01
Kyte-Doolittle Hydrophobicity Value -0.257
Molecular Weight (kDa) 72.6
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4I3M SIGNALING PROTEIN 11/26/12 Aer2 poly-HAMP domains: L44H HAMP1 CW-lock mutant PSEUDOMONAS AERUGINOSA 1.95 X-RAY DIFFRACTION 99.4
3LNR SIGNALING PROTEIN 02/02/10 Crystal structure of poly-HAMP domains from the P. aeruginosa soluble receptor Aer2 PSEUDOMONAS AERUGINOSA 2.64 X-RAY DIFFRACTION 100.0
4I44 SIGNALING PROTEIN 11/27/12 Aer2 poly-HAMP domains: V33G HAMP1 inverted signaling mutant PSEUDOMONAS AERUGINOSA 2.88 X-RAY DIFFRACTION 99.4
3VOL SIGNALING PROTEIN 01/27/12 X-ray Crystal Structure of PAS-HAMP Aer2 in the CN-bound Form PSEUDOMONAS AERUGINOSA 2.399 X-RAY DIFFRACTION 95.5
4HI4 SIGNALING PROTEIN 10/11/12 Crystal structure of the 5-coordinate ferric heme-binding PAS domain of Aer2 from P. aeruginosa PSEUDOMONAS AERUGINOSA 2.304 X-RAY DIFFRACTION 95.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 398 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000174 (401 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0176
Search term: aer2

References

The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa.
Hong CS, Kuroda A, Ikeda T, Takiguchi N, Ohtake H, Kato J
J. Biosci. Bioeng. 2004 ;97(3):184-90
PubMed ID: 16233612
Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa.
Hong CS, Shitashiro M, Kuroda A, Ikeda T, Takiguchi N, Ohtake H, Kato J
FEMS Microbiol. Lett. 2004 Feb;231(2):247-52
PubMed ID: 14987771
Sites of deamidation and methylation in Tsr, a bacterial chemotaxis sensory transducer.
Rice MS, Dahlquist FW
J. Biol. Chem. 1991 May;266(15):9746-53
PubMed ID: 2033064