Pseudomonas aeruginosa PAO1, PA0652 (vfr)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0652
Name
vfr
Replicon chromosome
Genomic location 706028 - 706672 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249343.1
GI 15595849
Affymetrix PA0652_vfr_at
DNASU PaCD00006293
Entrez 880744
GenBank AAG04041.1
INSDC AAG04041.1
NCBI Locus Tag PA0652
protein_id(GenBank) gb|AAG04041.1|AE004500_7|gnl|PseudoCAP|PA0652
TIGR NTL03PA00653
UniParc UPI0000138482
UniProtKB Acc P55222
UniProtKB ID VFR_PSEAE
UniRef100 UniRef100_P55222
UniRef50 UniRef50_P29281
UniRef90 UniRef90_P55222

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator Vfr
Product Name Confidence: Class 1
Synonyms cyclic AMP receptor-like protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.29
Kyte-Doolittle Hydrophobicity Value -0.250
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 8.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2OZ6 DNA BINDING PROTEIN 02/24/07 Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Not available / Not known
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse model of acute pneumonia
Reference 14977975

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 443 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000272 (995 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0652
Search term: vfr

References

Vfr controls quorum sensing in Pseudomonas aeruginosa.
Albus AM, Pesci EC, Runyen-Janecky LJ, West SE, Iglewski BH
J Bacteriol 1997 Jun;179(12):3928-35
PubMed ID: 9190808
The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family.
West SE, Sample AK, Runyen-Janecky LJ
J. Bacteriol. 1994 Dec;176(24):7532-42
PubMed ID: 8002577