Pseudomonas aeruginosa PAO1, PA0710 (gloA2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0710
Name
gloA2
Replicon chromosome
Genomic location 782570 - 782965 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249401.1
GI 15595907
Affymetrix PA0710_gloA2_at
DNASU PaCD00006326
Entrez 878796
GenBank AAG04099.1
INSDC AAG04099.1
NCBI Locus Tag PA0710
protein_id(GenBank) gb|AAG04099.1|AE004506_9|gnl|PseudoCAP|PA0710
TIGR NTL03PA00711
UniParc UPI00000C5113
UniProtKB Acc Q9I5L8
UniProtKB ID Q9I5L8_PSEAE
UniRef100 UniRef100_Q9I5L8
UniRef50 UniRef50_W6M5E2
UniRef90 UniRef90_Q9I5L8

Product

Feature Type CDS
Coding Frame 1
Product Name
lactoylglutathione lyase
Product Name Confidence: Class 1
Synonyms glyoxalase I
Evidence for Translation
Charge (pH 7) -3.08
Kyte-Doolittle Hydrophobicity Value -0.363
Molecular Weight (kDa) 14.9
Isoelectric Point (pI) 5.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4MTS LYASE 09/20/13 Ni- and Zn-bound GloA2 at high resolution PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
4MTQ LYASE 09/20/13 Ni-bound GloA2 PSEUDOMONAS AERUGINOSA 2.17 X-RAY DIFFRACTION 100.0
4MTT LYASE 09/20/13 Ni- and Zn-bound GloA2 at low resolution PSEUDOMONAS AERUGINOSA 2.17 X-RAY DIFFRACTION 100.0
4MTR LYASE 09/20/13 Zn-bound GloA2 PSEUDOMONAS AERUGINOSA 1.83 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 324 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000687 (304 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0710
Search term: gloA2

References

Isolation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences.
Clugston SL, Daub E, Kinach R, Miedema D, Barnard JF, Honek JF
Gene 1997 Feb;186(1):103-11
PubMed ID: 9047352
Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes.
Sukdeo N, Honek JF
Biochim. Biophys. Acta 2007 Jun;1774(6):756-63
PubMed ID: 17513180