Pseudomonas aeruginosa PAO1, PA0995 (ogt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0995
Name
ogt
Replicon chromosome
Genomic location 1077383 - 1077904 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249686.1
GI 15596192
Affymetrix PA0995_ogt_at
DNASU PaCD00006459
Entrez 880794
GenBank AAG04384.1
INSDC AAG04384.1
NCBI Locus Tag PA0995
protein_id(GenBank) gb|AAG04384.1|AE004532_8|gnl|PseudoCAP|PA0995
TIGR NTL03PA00996
UniParc UPI00000C51F7
UniProtKB Acc Q9I4X4
UniProtKB ID Q9I4X4_PSEAE
UniRef100 UniRef100_W0YPL0
UniRef50 UniRef50_Q6FEA2
UniRef90 UniRef90_B7UXU8

Product

Feature Type CDS
Coding Frame 1
Product Name
methylated-DNA--protein-cysteine methyltransferase
Product Name Confidence: Class 2
Synonyms O-6-alkylguanine-DNA/cysteine-protein methyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.69
Kyte-Doolittle Hydrophobicity Value -0.261
Molecular Weight (kDa) 19.3
Isoelectric Point (pI) 7.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 571 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000958 (494 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0995
Search term: ogt

References

Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli.
Potter PM, Wilkinson MC, Fitton J, Carr FJ, Brennand J, Cooper DP, Margison GP
Nucleic Acids Res 1987 Nov 25;15(22):9177-93
PubMed ID: 2825131
Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein.
Harris LC, Potter PM, Margison GP
Biochem Biophys Res Commun 1992 Aug 31;187(1):425-31
PubMed ID: 1520330