Pseudomonas aeruginosa PAO1, PA1288

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1288
Name
Synonym: fadL
Synonym: ompP1
Replicon chromosome
Genomic location 1399231 - 1400505 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_249979.1
GI 15596485
Affymetrix PA1288_at
Entrez 881473
GenBank AAG04677.1
INSDC AAG04677.1
NCBI Locus Tag PA1288
protein_id(GenBank) gb|AAG04677.1|AE004558_6|gnl|PseudoCAP|PA1288
TIGR NTL03PA01289
UniParc UPI00000C52F0
UniProtKB Acc Q9I456
UniProtKB ID Q9I456_PSEAE
UniRef100 UniRef100_A0A069Q437
UniRef50 UniRef50_A0A069Q437
UniRef90 UniRef90_A0A069Q437

Product

Feature Type CDS
Coding Frame 1
Product Name
probable outer membrane protein precursor
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (45.56 kDa, PMID:21751344)
Charge (pH 7) -3.56
Kyte-Doolittle Hydrophobicity Value -0.263
Molecular Weight (kDa) 45.6
Isoelectric Point (pI) 5.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 2
Outer Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 203 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001233 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1288

References

Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli.
Said B, Ghosn CR, Vu L, Nunn WD
Mol Microbiol 1988 May;2(3):363-70
PubMed ID: 2840553
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878