Pseudomonas aeruginosa PAO1, PA1337 (ansB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1337
Name
ansB
Synonym: pga
Replicon chromosome
Genomic location 1450545 - 1451633 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250028.1
GI 15596534
Affymetrix PA1337_ansB_at
DNASU PaCD00006616
Entrez 882207
GenBank AAG04726.1
INSDC AAG04726.1
NCBI Locus Tag PA1337
protein_id(GenBank) gb|AAG04726.1|AE004563_3|gnl|PseudoCAP|PA1337
TIGR NTL03PA01338
UniParc UPI00000C5320
UniProtKB Acc Q9I407
UniProtKB ID ASPQ_PSEAE
UniRef100 UniRef100_Q9I407
UniRef50 UniRef50_P10182
UniRef90 UniRef90_Q9I407

Product

Feature Type CDS
Coding Frame 1
Product Name
glutaminase-asparaginase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.68
Kyte-Doolittle Hydrophobicity Value -0.020
Molecular Weight (kDa) 38.6
Isoelectric Point (pI) 7.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 351 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001273 (467 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1337
Search term: ansB

References

Amino acid sequence of the diazooxonorleucine binding site of Acinetobacter and Pseudomonas 7A glutaminase--asparaginase enzymes.
Holcenberg JS, Ericsson L, Roberts J
Biochemistry 1978 Feb 7;17(3):411-7
PubMed ID: 619999
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994