Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5112
|
Name |
estA
|
Replicon | chromosome |
Genomic location | 5754298 - 5756238 (- strand) |
Transposon Mutants | 7 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_253799.1 |
GI | 15600305 |
Affymetrix | PA5112_estA_at |
Entrez | 878826 |
GenBank | AAG08497.1 |
INSDC | AAG08497.1 |
NCBI Locus Tag | PA5112 |
protein_id(GenBank) | gb|AAG08497.1|AE004924_2|gnl|PseudoCAP|PA5112 |
TIGR | NTL03PA05113 |
UniParc | UPI00000D426D |
UniProtKB Acc | O33407 |
UniProtKB ID | ESTA_PSEAE |
UniRef100 | UniRef100_O33407 |
UniRef50 | UniRef50_O33407 |
UniRef90 | UniRef90_O33407 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
esterase EstA
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (69.61 kDa, PMID:21751344)
|
Charge (pH 7) | -21.63 |
Kyte-Doolittle Hydrophobicity Value | -0.375 |
Molecular Weight (kDa) | 69.6 |
Isoelectric Point (pI) | 4.46 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3KVN | HYDROLASE | 11/30/09 | Crystal structure of the full-length autotransporter EstA from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.499 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
(Hydroxyethyloxy)Tri(Ethyloxy)Octane | DB04233 | DrugBank | 5.1.4 | O33407 | Esterase EstA | 100.0 | 646 | 0.0e+00 |
Source 1 | |
Database | Victors : 4222 |
Category | |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Rattus norvegicus |
Notes | |
Infection model | Rat agar bead model of chronic lung infection |
Reference | 14641575 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004538 (814 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5112
Search term: estA
Search term: esterase EstA
|
A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa.
Wilhelm S, Tommassen J, Jaeger KE
J Bacteriol 1999 Nov;181(22):6977-86
PubMed ID: 10559163
|
Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC.
Essar DW, Eberly L, Crawford IP
J Bacteriol 1990 Feb;172(2):867-83
PubMed ID: 2404959
|
Extracellular enzymes affect biofilm formation of mucoid Pseudomonas aeruginosa.
Tielen P, Rosenau F, Wilhelm S, Jaeger KE, Flemming HC, Wingender J
Microbiology (Reading) 2010 Jul;156(Pt 7):2239-2252
PubMed ID: 20360178
|
The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation.
Wilhelm S, Gdynia A, Tielen P, Rosenau F, Jaeger KE
J Bacteriol 2007 Sep;189(18):6695-703
PubMed ID: 17631636
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|