Pseudomonas aeruginosa PAO1, PA5490 (cc4)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5490
Name
cc4
Replicon chromosome
Genomic location 6181745 - 6182350 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254177.1
GI 15600683
Affymetrix PA5490_cc4_at
DNASU PaCD00008365
Entrez 877732
GenBank AAG08875.1
INSDC AAG08875.1
NCBI Locus Tag PA5490
protein_id(GenBank) gb|AAG08875.1|AE004961_10|gnl|PseudoCAP|PA5490
TIGR NTL03PA05491
UniParc UPI0000128B50
UniProtKB Acc P00106
UniProtKB ID CYC4_PSEAE
UniRef100 UniRef100_P00106
UniRef50 UniRef50_P00106
UniRef90 UniRef90_P00106

Product

Feature Type CDS
Coding Frame 1
Product Name
cytochrome c4 precursor
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (20.73 kDa, PMID:21751344)
Charge (pH 7) 1.55
Kyte-Doolittle Hydrophobicity Value -0.047
Molecular Weight (kDa) 20.7
Isoelectric Point (pI) 7.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 144 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005030 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5490
Search term: cc4

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and characterisation of the gene encoding cytochrome c4 from Pseudomonas stutzeri.
Christensen HE
Gene 1994 Jun;144(1):139-40
PubMed ID: 8026750
Crystal structure of the dihaem cytochrome c4 from Pseudomonas stutzeri determined at 2.2A resolution.
Kadziola A, Larsen S
Structure 1997 Feb;5(2):203-16
PubMed ID: 9032080
Cytochrome c terminal oxidase pathways of Azotobacter vinelandii: analysis of cytochrome c4 and c5 mutants and up-regulation of cytochrome c-dependent pathways with N2 fixation.
Rey L, Maier RJ
J. Bacteriol. 1997 Nov;179(22):7191-6
PubMed ID: 9371471
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344