Pseudomonas aeruginosa PAO1, PA0020 (tsaP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0020
Name
tsaP
Replicon chromosome
Genomic location 21712 - 22737 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248710.1
GI 15595218
Affymetrix PA0020_at
DNASU PaCD00006012
Entrez 879309
GenBank AAG03410.1
INSDC AAG03410.1
NCBI Locus Tag PA0020
protein_id(GenBank) gb|AAG03410.1|AE004441_11|gnl|PseudoCAP|PA0020
TIGR NTL03PA00021
UniParc UPI00000C4EED
UniProtKB Acc Q9I7A7
UniProtKB ID Q9I7A7_PSEAE
UniRef100 UniRef100_W0YJF2
UniRef50 UniRef50_A6UX82
UniRef90 UniRef90_A6UX82

Product

Feature Type CDS
Coding Frame 1
Product Name
T4P secretin-associated protein TsaP
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 3.73
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 37.6
Isoelectric Point (pI) 9.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 108 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000020 (480 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0020
Search term: tsaP

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Peptidoglycan-binding protein TsaP functions in surface assembly of type IV pili.
Siewering K, Jain S, Friedrich C, Webber-Birungi MT, Semchonok DA, Binzen I, Wagner A, Huntley S, Kahnt J, Klingl A, Boekema EJ, Søgaard-Andersen L, van der Does C
Proc. Natl. Acad. Sci. U.S.A. 2014 Mar;111(10):E953-61
PubMed ID: 24556993