Pseudomonas aeruginosa PAO1, PA0768 (lepB)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: lep
Synonym: SPASE I
Replicon chromosome
Genomic location 837328 - 838182 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1


RefSeq NP_249459.1
GI 15595965
Affymetrix PA0768_lepB_at
Entrez 882104
GenBank AAG04157.1
INSDC AAG04157.1
NCBI Locus Tag PA0768
protein_id(GenBank) gb|AAG04157.1|AE004511_10|gnl|PseudoCAP|PA0768
UniParc UPI000012E4A6
UniProtKB Acc Q9I5G7
UniRef100 UniRef100_Q9I5G7
UniRef50 UniRef50_Q9I5G7
UniRef90 UniRef90_Q9I5G7


Feature Type CDS
Coding Frame 1
Product Name
signal peptidase I
Product Name Confidence: Class 1
Synonyms leader peptidase 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (32.10 kDa, PMID:21751344)
Charge (pH 7) 2.69
Kyte-Doolittle Hydrophobicity Value -0.067
Molecular Weight (kDa) 32.1
Isoelectric Point (pI) 8.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 569 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000742 (552 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0768
Search term: lepB


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.
Wolfe PB, Wickner W, Goodman JM
J Biol Chem 1983 Oct 10;258(19):12073-80
PubMed ID: 6311837
On the catalytic mechanism of prokaryotic leader peptidase 1.
Black MT, Munn JG, Allsop AE
Biochem J 1992 Mar 1;282 ( Pt 2):539-43
PubMed ID: 1546969
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Pseudomonas aeruginosa possesses two putative type I signal peptidases, LepB and PA1303, each with distinct roles in physiology and virulence.
Waite RD, Rose RS, Rangarajan M, Aduse-Opoku J, Hashim A, Curtis MA
J. Bacteriol. 2012 Sep;194(17):4521-36
PubMed ID: 22730125