Pseudomonas aeruginosa PAO1, PA0861 (rbdA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0861
Name
rbdA
Replicon chromosome
Genomic location 940117 - 942573 (+ strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_249552.1
GI 15596058
Affymetrix PA0861_at
Entrez 882259
GenBank AAG04250.1
INSDC AAG04250.1
NCBI Locus Tag PA0861
protein_id(GenBank) gb|AAG04250.1|AE004521_1|gnl|PseudoCAP|PA0861
TIGR NTL03PA00862
UniParc UPI00000C5193
UniProtKB Acc Q9I580
UniProtKB ID Q9I580_PSEAE
UniRef100 UniRef100_Q9I580
UniRef50 UniRef50_D7I2P9
UniRef90 UniRef90_Q9I580

Product

Feature Type CDS
Coding Frame 1
Product Name
RbDA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -25.63
Kyte-Doolittle Hydrophobicity Value -0.070
Molecular Weight (kDa) 91.2
Isoelectric Point (pI) 4.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5XGE TRANSCRIPTION 04/13/17 Crystal structure of the PAS-GGDEF-EAL domain of PA0861 from Pseudomonas aeruginosa in complex with cyclic di-GMP PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.31 X-RAY DIFFRACTION 100.0
5XGB TRANSCRIPTION 04/13/17 Crystal structure of the PAS-GGDEF-EAL domain of PA0861 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.28 X-RAY DIFFRACTION 100.0
5XGD TRANSCRIPTION 04/13/17 Crystal structure of the PAS-GGDEF-EAL domain of PA0861 from Pseudomonas aeruginosa in complex with GTP PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 477 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000828 (501 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0861
Search term: rbdA
Search term: RbDA

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The phosphodiesterase DipA (PA5017) is essential for Pseudomonas aeruginosa biofilm dispersion.
Roy AB, Petrova OE, Sauer K
J. Bacteriol. 2012 Jun;194(11):2904-15
PubMed ID: 22493016