Pseudomonas aeruginosa PAO1, PA1003 (mvfR)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: pqsR
Replicon chromosome
Genomic location 1086097 - 1087095 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentQuinolone signal response


RefSeq NP_249694.1
GI 15596200
Affymetrix PA1003_at
DNASU PaCD00006463
Entrez 879994
GenBank AAG04392.1
INSDC AAG04392.1
NCBI Locus Tag PA1003
protein_id(GenBank) gb|AAG04392.1|AE004533_3|gnl|PseudoCAP|PA1003
UniParc UPI00000C51FB
UniProtKB Acc Q9I4X0
UniRef100 UniRef100_W0YN21
UniRef50 UniRef50_Q02IG8
UniRef90 UniRef90_Q02IG8


Feature Type CDS
Coding Frame 1
Product Name
Transcriptional regulator MvfR
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 1.77
Kyte-Doolittle Hydrophobicity Value -0.138
Molecular Weight (kDa) 37.2
Isoelectric Point (pI) 7.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4JVI TRANSCRIPTION REGULATOR/INHIBITOR 03/25/13 Crystal structure of PqsR co-inducer binding domain of Pseudomonas aeruginosa with inhibitor 3NH2-7Cl-C9QZN PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
6B8A TRANSCRIPTION 10/05/17 Crystal structure of MvfR ligand binding domain in complex with M64 PSEUDOMONAS AERUGINOSA 2.65 X-RAY DIFFRACTION 100.0
4JVD TRANSCRIPTION REGULATOR 03/25/13 Crystal structure of PqsR coinducer binding domain of Pseudomonas aeruginosa with ligand NHQ PSEUDOMONAS AERUGINOSA 2.95 X-RAY DIFFRACTION 100.0
4JVC TRANSCRIPTION REGULATOR 03/25/13 Crystal structure of PqsR co-inducer binding domain PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 139 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000966 (294 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1003
Search term: mvfR


A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism.
Cao H, Krishnan G, Goumnerov B, Tsongalis J, Tompkins R, Rahme LG
Proc Natl Acad Sci U S A 2001 Dec 4;98(25):14613-8
PubMed ID: 11724939
Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication.
Déziel E, Lépine F, Milot S, He J, Mindrinos MN, Tompkins RG, Rahme LG
Proc Natl Acad Sci U S A 2004 Feb 3;101(5):1339-44
PubMed ID: 14739337
The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation: multiple quorum sensing-regulated genes are modulated without affecting lasRI, rhlRI or the production of N-acyl-L-homoserine lactones.
Déziel E, Gopalan S, Tampakaki AP, Lépine F, Padfield KE, Saucier M, Xiao G, Rahme LG
Mol Microbiol 2005 Feb;55(4):998-1014
PubMed ID: 15686549