Pseudomonas aeruginosa PAO1, PA2493 (mexE)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA2493
|
| Name |
mexE
|
| Replicon | chromosome |
| Genomic location | 2808743 - 2809987 (+ strand) |
| Transposon Mutants | 3 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_251183.1 |
| GI | 15597689 |
| Affymetrix | PA2493_mexE_at |
| Entrez | 880212 |
| GenBank | AAG05881.1 |
| INSDC | AAG05881.1 |
| NCBI Locus Tag | PA2493 |
| protein_id(GenBank) | gb|AAG05881.1|AE004676_13|gnl|PseudoCAP|PA2493 |
| TIGR | NTL03PA02493 |
| UniParc | UPI00000C5703 |
| UniProtKB Acc | Q9I0Y9 |
| UniProtKB ID | Q9I0Y9_PSEAE |
| UniRef100 | UniRef100_W0YVG8 |
| UniRef50 | UniRef50_W0YVG8 |
| UniRef90 | UniRef90_W0YVG8 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
Resistance-Nodulation-Cell Division (RND) multidrug efflux membrane fusion protein MexE precursor
|
| Synonyms |
RND multidrug efflux membrane fusion protein MexE precursor |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 2.71 |
| Kyte-Doolittle Hydrophobicity Value | -0.350 |
| Molecular Weight (kDa) | 45.0 |
| Isoelectric Point (pI) | 8.84 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AMR gene predictions from CARD database
Identified using the Resistance Gene Identifier (RGI)
| Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
| MexE | MexE is the membrane fusion protein of the MexEF-OprN multidrug efflux complex. [PMID:10515918] | ARO:3000803 | protein homolog model | Perfect | 785.4 | 100.0 | 3.2.4 |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 414 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG003063 (543 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA2493
Search term: mexE
|
Human Homologs
References
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol. Microbiol. 1997 Jan;23(2):345-54
PubMed ID: 9044268
|