Pseudomonas aeruginosa PAO1, PA2493 (mexE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 2808743 - 2809987 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_251183.1
GI 15597689
Affymetrix PA2493_mexE_at
Entrez 880212
GenBank AAG05881.1
INSDC AAG05881.1
NCBI Locus Tag PA2493
protein_id(GenBank) gb|AAG05881.1|AE004676_13|gnl|PseudoCAP|PA2493
UniParc UPI00000C5703
UniProtKB Acc Q9I0Y9
UniRef100 UniRef100_W0YVG8
UniRef50 UniRef50_W0YVG8
UniRef90 UniRef90_W0YVG8


Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux membrane fusion protein MexE precursor
Product Name Confidence: Class 1
Synonyms RND multidrug efflux membrane fusion protein MexE precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.71
Kyte-Doolittle Hydrophobicity Value -0.350
Molecular Weight (kDa) 45.0
Isoelectric Point (pI) 8.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexE MexE is the membrane fusion protein of the MexEF-OprN multidrug efflux complex. [PMID:10515918] ARO:3000803 protein homolog model Perfect 785.4 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 414 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003063 (526 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2493
Search term: mexE

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol Microbiol 1997 Jan;23(2):345-54
PubMed ID: 9044268