Pseudomonas aeruginosa PAO1, PA3158 (wbpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3158
Name
wbpB
Replicon chromosome
Genomic location 3542740 - 3543690 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251848.1
GI 15598354
Affymetrix PA3158_wbpB_at
DNASU PaCD00007389
Entrez 882642
GenBank AAG06546.1
INSDC AAG06546.1
NCBI Locus Tag PA3158
protein_id(GenBank) gb|AAG06546.1|AE004739_8|gnl|PseudoCAP|PA3158
TIGR NTL03PA03158
UniParc UPI00000DA0FF
UniProtKB Acc G3XD23
UniProtKB ID WBPB_PSEAE
UniRef100 UniRef100_G3XD23
UniRef50 UniRef50_G3XD23
UniRef90 UniRef90_G3XD23

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-2-acetamido-2-deoxy-d-glucuronic acid 3-dehydrogenase, WbpB
Product Name Confidence: Class 1
Synonyms UDP-2-acetamido-2-deoxy-d-glucuronic acid 3-dehydrogenase, WbpB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.03
Kyte-Doolittle Hydrophobicity Value -0.266
Molecular Weight (kDa) 35.7
Isoelectric Point (pI) 6.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3OA2 OXIDOREDUCTASE 08/04/10 Crystal structure of the WlbA (WbpB) dehydrogenase from Pseudomonas aeruginosa in complex with NAD at 1.5 angstrom resolution PSEUDOMONAS AERUGINOSA 1.5 X-RAY DIFFRACTION 99.7

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 201 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000236 (924 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3158
Search term: wbpB

Human Homologs

References

Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Burrows LL, Charter DF, Lam JS
Mol. Microbiol. 1996 Nov;22(3):481-95
PubMed ID: 8939432
Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa.
Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS
J. Biol. Chem. 2009 May;284(18):11854-62
PubMed ID: 19282284