Pseudomonas aeruginosa PAO1, PA0008 (glyS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0008
Name
glyS
Replicon chromosome
Genomic location 10434 - 12488 (- strand)

Cross-References

RefSeq NP_064728.1
GI 15595206
Affymetrix PA0008_glyS_at
Entrez 878214
GenBank AAG03398.1
INSDC AAG03398.1
NCBI Locus Tag PA0008
protein_id(GenBank) gb|AAG03398.1|AE004440_8|gnl|PseudoCAP|PA0008
TIGR NTL03PA00009
UniParc UPI00001364A2
UniProtKB Acc Q9I7B8
UniProtKB ID SYGB_PSEAE
UniRef100 UniRef100_Q9I7B8
UniRef50 UniRef50_Q4KKR8
UniRef90 UniRef90_Q9I7B8

Product

Feature Type CDS
Coding Frame 1
Product Name
glycyl-tRNA synthetase beta chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -15.14
Kyte-Doolittle Hydrophobicity Value -0.032
Molecular Weight (kDa) 74.0
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 403 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000008 (555 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0008
Search term: glyS

Human Homologs

References

Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame.
Keng T, Webster TA, Sauer RT, Schimmel P
J Biol Chem 1982 Nov 10;257(21):12503-8
PubMed ID: 6290471
Primary structures of both subunits of Escherichia coli glycyl-tRNA synthetase.
Webster TA, Gibson BW, Keng T, Biemann K, Schimmel P
J Biol Chem 1983 Sep 10;258(17):10637-41
PubMed ID: 6309809
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994