Pseudomonas aeruginosa PAO1, PA3624 (pcm)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3624
Name
pcm
Replicon chromosome
Genomic location 4059957 - 4060592 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252314.1
GI 15598820
Affymetrix PA3624_pcm_at
DNASU PaCD00007586
Entrez 880441
GenBank AAG07012.1
INSDC BAA05129.1
NCBI Locus Tag PA3624
protein_id(GenBank) gb|AAG07012.1|AE004782_10|gnl|PseudoCAP|PA3624
TIGR NTL03PA03624
UniParc UPI0000131AE7
UniProtKB Acc P45683
UniProtKB ID PIMT_PSEAE
UniRef100 UniRef100_B7V8C3
UniRef50 UniRef50_A6V1G4
UniRef90 UniRef90_A6V1G4

Product

Feature Type CDS
Coding Frame 1
Product Name
L-isoaspartate protein carboxylmethyltransferase type II
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.04
Kyte-Doolittle Hydrophobicity Value -0.027
Molecular Weight (kDa) 23.4
Isoelectric Point (pI) 6.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 418 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002050 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3624
Search term: pcm

Human Homologs

Ensembl 110, assembly GRCh38.p14
protein-L-isoaspartate (D-aspartate) O-methyltransferase [Source:HGNC Symbol;Acc:HGNC:8728]
E-value: 5.8e-18
Percent Identity: 36.0
Ensembl 110, assembly GRCh38.p14
protein-L-isoaspartate (D-aspartate) O-methyltransferase [Source:HGNC Symbol;Acc:HGNC:8728]
E-value: 5.8e-18
Percent Identity: 36.0
Ensembl 110, assembly GRCh38.p14
protein-L-isoaspartate (D-aspartate) O-methyltransferase [Source:HGNC Symbol;Acc:HGNC:8728]
E-value: 5.8e-18
Percent Identity: 36.0

References

Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli.
Fu JC, Ding L, Clarke S
J Biol Chem 1991 Aug 5;266(22):14562-72
PubMed ID: 1860862
The L-isoaspartyl protein repair methyltransferase enhances survival of aging Escherichia coli subjected to secondary environmental stresses.
Visick JE, Cai H, Clarke S
J Bacteriol 1998 May;180(10):2623-9
PubMed ID: 9573145
Isoaspartate in ribosomal protein S11 of Escherichia coli.
David CL, Keener J, Aswad DW
J Bacteriol 1999 May;181(9):2872-7
PubMed ID: 10217780