Pseudomonas aeruginosa PAO1, PA3987 (leuS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Molecular Function GO:0004823 leucine-tRNA ligase activity
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000250
sequence similarity evidence used in manual assertion		 10024478 Reviewed by curator
Biological Process GO:0006418 tRNA aminoacylation for protein translation
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000250
sequence similarity evidence used in manual assertion		 10024478 Reviewed by curator
Biological Process GO:0006520 cellular amino acid metabolic process
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000044
sequence similarity evidence
Biological Process GO:0044267 cellular protein metabolic process
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000044
sequence similarity evidence
Molecular Function GO:0000166 nucleotide binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_00049_B
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0002161 aminoacyl-tRNA editing activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF13603
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Biological Process GO:0006429 leucyl-tRNA aminoacylation
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_00049_B
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0004812 aminoacyl-tRNA ligase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF09334
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Biological Process GO:0006418 tRNA aminoacylation for protein translation
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF09334
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0005524 ATP binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_00049_B
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0004823 leucine-tRNA ligase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_00049_B
 ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Translation, post-translational modification, degradation Other PAO1 genes in this class
Amino acid biosynthesis and metabolism Other PAO1 genes in this class

Pathways

Database Xref Pathway Version Evidence PMID
KEGG pae00970 Aminoacyl-tRNA biosynthesis 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
PseudoCyc TRNA-CHARGING-PWY tRNA charging 19.5 ECO:0000250
sequence similarity evidence used in manual assertion 		12867747
PseudoCAP Valine, leucine and isoleucine biosynthesis ECO:0000037
not_recorded
PseudoCAP Aminoacyl-tRNA biosynthesis ECO:0000037
not_recorded

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
SUPERFAMILY SSF52374 Nucleotidylyl transferase - - 5 681 0.0
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 185 198 6.8E-51
PANTHER PTHR43740 LEUCYL-TRNA SYNTHETASE IPR002302 Leucine-tRNA ligase 3 872 0.0
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 215 234 6.8E-51
Gene3D G3DSA:3.40.50.620 HUPs IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 32 228 6.9E-84
FunFam G3DSA:3.40.50.620:FF:000124 Leucine--tRNA ligase - - 365 583 1.1E-100
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 153 169 6.8E-51
Gene3D G3DSA:3.10.20.590 - - - 811 873 5.0E-23
MobiDBLite mobidb-lite consensus disorder prediction - - 624 643 -
Hamap MF_00049_B Leucine--tRNA ligase [leuS]. IPR002302 Leucine-tRNA ligase 4 872 23.377468
NCBIfam TIGR00396 JCVI: bacterial-type leucine--tRNA ligase IPR002302 Leucine-tRNA ligase 5 872 0.0
Gene3D G3DSA:1.10.730.10 - - - 6 31 6.9E-84
Pfam PF09334 tRNA synthetases class I (M) IPR015413 Methionyl/Leucyl tRNA synthetase 39 171 5.9E-19
CDD cd00812 LeuRS_core - - 34 235 1.42592E-87
Gene3D G3DSA:3.90.740.10 - IPR009008 Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain 236 409 1.1E-23
Pfam PF00133 tRNA synthetases class I (I, L, M and V) IPR002300 Aminoacyl-tRNA synthetase, class Ia 631 666 4.9E-7
Gene3D G3DSA:3.40.50.620 HUPs IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 410 580 2.5E-67
SUPERFAMILY SSF50677 ValRS/IleRS/LeuRS editing domain IPR009008 Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain 226 424 1.26E-49
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 497 515 6.8E-51
Gene3D G3DSA:1.10.730.10 - - - 638 810 1.4E-63
FunFam G3DSA:3.90.740.10:FF:000012 Leucine--tRNA ligase - - 239 419 2.3E-85
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 569 579 6.8E-51
FunFam G3DSA:1.10.730.10:FF:000003 Leucine--tRNA ligase - - 638 810 2.3E-79
FunFam G3DSA:3.40.50.620:FF:000003 Leucine--tRNA ligase - - 32 252 2.9E-111
Gene3D G3DSA:2.20.28.290 - - - 581 637 1.7E-28
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 536 558 6.8E-51
FunFam G3DSA:2.20.28.290:FF:000001 Leucine--tRNA ligase - - 581 637 1.6E-24
PRINTS PR00985 Leucyl-tRNA synthetase signature IPR002302 Leucine-tRNA ligase 127 144 6.8E-51
Pfam PF08264 Anticodon-binding domain of tRNA ligase IPR013155 Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding 714 834 1.3E-14
Pfam PF13603 Leucyl-tRNA synthetase, Domain 2 IPR025709 Leucyl-tRNA synthetase, editing domain 221 413 4.8E-68
CDD cd07958 Anticodon_Ia_Leu_BEm - - 671 789 8.32098E-45
Pfam PF00133 tRNA synthetases class I (I, L, M and V) IPR002300 Aminoacyl-tRNA synthetase, class Ia 428 584 5.5E-8
SUPERFAMILY SSF47323 Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases IPR009080 Aminoacyl-tRNA synthetase, class Ia, anticodon-binding 675 872 9.53E-34
FunFam G3DSA:3.10.20.590:FF:000001 Leucine--tRNA ligase - - 811 873 1.1E-25

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.