Pseudomonas aeruginosa PAO1, PA0548 (tktA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0548
Name
tktA
Replicon chromosome
Genomic location 606160 - 608157 (+ strand)

Cross-References

RefSeq NP_249239.1
GI 15595745
Affymetrix PA0548_tktA_at
Entrez 878523
GenBank AAG03937.1
INSDC AAG03937.1
NCBI Locus Tag PA0548
protein_id(GenBank) gb|AAG03937.1|AE004491_4|gnl|PseudoCAP|PA0548
TIGR NTL03PA00549
UniParc UPI00000C50AB
UniProtKB Acc Q9I5Y8
UniProtKB ID Q9I5Y8_PSEAE
UniRef100 UniRef100_Q9I5Y8
UniRef50 UniRef50_P22976
UniRef90 UniRef90_Q9I5Y8

Product

Feature Type CDS
Coding Frame 1
Product Name
transketolase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -23.37
Kyte-Doolittle Hydrophobicity Value -0.212
Molecular Weight (kDa) 72.2
Isoelectric Point (pI) 4.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4XEU TRANSFERASE 12/24/14 Crystal structure of a transketolase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.95 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 608 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000532 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0548
Search term: tktA
Search term: transketolase

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene.
Sprenger GA
Biochim. Biophys. Acta 1993 Nov;1216(2):307-10
PubMed ID: 8241274
Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains.
Sprenger GA, Schörken U, Sprenger G, Sahm H
Eur. J. Biochem. 1995 Jun;230(2):525-32
PubMed ID: 7607225
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994