Pseudomonas aeruginosa PAO1, PA1094 (fliD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1094
Name
fliD
Replicon chromosome
Genomic location 1185060 - 1186484 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249785.1
GI 15596291
Affymetrix PA1094_fliD_at
Entrez 881775
GenBank AAG04483.1
INSDC AAA99809.1
NCBI Locus Tag PA1094
protein_id(GenBank) gb|AAG04483.1|AE004540_3|gnl|PseudoCAP|PA1094
TIGR NTL03PA01095
UniParc UPI000012A8EA
UniProtKB Acc Q9K3C5
UniProtKB ID FLID2_PSEAE
UniRef100 UniRef100_Q9K3C5
UniRef50 UniRef50_Q9K3C5
UniRef90 UniRef90_Q9K3C5

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellar capping protein FliD
Product Name Confidence: Class 1
Synonyms hook-associated protein
Evidence for Translation
Detected using LC-MS/MS (49.45 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.01
Kyte-Doolittle Hydrophobicity Value -0.223
Molecular Weight (kDa) 49.4
Isoelectric Point (pI) 7.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Flagellar
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Extracellular Class 3
Flagellar Class 1
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5FHY STRUCTURAL PROTEIN 12/22/15 Crystal structure of FliD (HAP2) from Pseudomonas aeruginosa PAO1 PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.201 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 252 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001055 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1094
Search term: fliD

References

Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes.
Kawagishi I, Müller V, Williams AW, Irikura VM, Macnab RM
J. Gen. Microbiol. 1992 Jun;138(6):1051-65
PubMed ID: 1527488
The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion.
Arora SK, Ritchings BW, Almira EC, Lory S, Ramphal R
Infect. Immun. 1998 Mar;66(3):1000-7
PubMed ID: 9488388
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344