Pseudomonas aeruginosa PAO1, PA1249 (aprA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1249
Name
aprA
Replicon chromosome
Genomic location 1355631 - 1357070 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249940.1
GI 15596446
Affymetrix PA1249_aprA_at
Entrez 881248
GenBank AAG04638.1
INSDC AAG04638.1
NCBI Locus Tag PA1249
protein_id(GenBank) gb|AAG04638.1|AE004554_6|gnl|PseudoCAP|PA1249
TIGR NTL03PA01250
UniParc UPI00001120E0
UniProtKB Acc Q03023
UniProtKB ID APRA_PSEAE
UniRef100 UniRef100_Q03023
UniRef50 UniRef50_Q03023
UniRef90 UniRef90_Q03023

Product

Feature Type CDS
Coding Frame 1
Product Name
alkaline metalloproteinase precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -28.31
Kyte-Doolittle Hydrophobicity Value -0.223
Molecular Weight (kDa) 50.4
Isoelectric Point (pI) 4.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1AKL HYDROLASE (METALLOPROTEINASE) 09/16/95 ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1KAP ZINC METALLOPROTEASE 06/08/95 THREE-DIMENSIONAL STRUCTURE OF THE ALKALINE PROTEASE OF PSEUDOMONAS AERUGINOSA: A TWO-DOMAIN PROTEIN WITH A CALCIUM BINDING PARALLEL BETA ROLL MOTIF PSEUDOMONAS AERUGINOSA 1.64 X-RAY DIFFRACTION 100.0
1JIW HYDROLASE/HYDROLASE INHIBITOR 07/03/01 Crystal structure of the APR-APRin complex PSEUDOMONAS AERUGINOSA 1.74 X-RAY DIFFRACTION 100.0
3VI1 HYDROLASE 09/13/11 Crystal structure of Pseudomonas aerginosa alkaline protease complexed with Substance P(1-6) PSEUDOMONAS AERUGINOSA; SYNTHETIC CONSTRUCT 2.0 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0090
Category Alkaline protease
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 127 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001201 (1306 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1249
Search term: aprA

References

Quantitation and enzymatic activity of the alkaline protease from Pseudomonas aeruginosa in culture supernatants from clinical strains.
Louis D, Sorlier P, Wallach J
Clin. Chem. Lab. Med. 1998 May;36(5):295-8
PubMed ID: 9676386
Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.
Okuda K, Morihara K, Atsumi Y, Takeuchi H, Kawamoto S, Kawasaki H, Suzuki K, Fukushima J
Infect. Immun. 1990 Dec;58(12):4083-8
PubMed ID: 2123832
Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding.
Miyatake H, Hata Y, Fujii T, Hamada K, Morihara K, Katsube Y
J. Biochem. 1995 Sep;118(3):474-9
PubMed ID: 8690704
Pseudomonas aeruginosa alkaline protease: evidence for secretion genes and study of secretion mechanism.
Guzzo J, Pages JM, Duong F, Lazdunski A, Murgier M
J. Bacteriol. 1991 Sep;173(17):5290-7
PubMed ID: 1832151