Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1249
|
Name |
aprA
|
Replicon | chromosome |
Genomic location | 1355631 - 1357070 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
RefSeq | NP_249940.1 |
GI | 15596446 |
Affymetrix | PA1249_aprA_at |
Entrez | 881248 |
GenBank | AAG04638.1 |
INSDC | AAG04638.1 |
NCBI Locus Tag | PA1249 |
protein_id(GenBank) | gb|AAG04638.1|AE004554_6|gnl|PseudoCAP|PA1249 |
TIGR | NTL03PA01250 |
UniParc | UPI00001120E0 |
UniProtKB Acc | Q03023 |
UniProtKB ID | APRA_PSEAE |
UniRef100 | UniRef100_Q03023 |
UniRef50 | UniRef50_Q03023 |
UniRef90 | UniRef90_Q03023 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
alkaline metalloproteinase precursor
|
Synonyms | |
Evidence for Translation | |
Charge (pH 7) | -28.31 |
Kyte-Doolittle Hydrophobicity Value | -0.223 |
Molecular Weight (kDa) | 50.4 |
Isoelectric Point (pI) | 4.09 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1KAP | ZINC METALLOPROTEASE | 06/08/95 | THREE-DIMENSIONAL STRUCTURE OF THE ALKALINE PROTEASE OF PSEUDOMONAS AERUGINOSA: A TWO-DOMAIN PROTEIN WITH A CALCIUM BINDING PARALLEL BETA ROLL MOTIF | Pseudomonas aeruginosa | 1.64 | X-RAY DIFFRACTION | 100.0 |
1JIW | HYDROLASE/HYDROLASE INHIBITOR | 07/03/01 | Crystal structure of the APR-APRin complex | Pseudomonas aeruginosa | 1.74 | X-RAY DIFFRACTION | 100.0 |
1AKL | HYDROLASE (METALLOPROTEINASE) | 09/16/95 | ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080 | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
3VI1 | HYDROLASE | 09/13/11 | Crystal structure of Pseudomonas aerginosa alkaline protease complexed with Substance P(1-6) | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 2 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | VFDB : VF0090 |
Category | Alkaline protease |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 127 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001201 (1272 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1249
Search term: aprA
Search term: alkaline metalloproteinase precursor
|
Quantitation and enzymatic activity of the alkaline protease from Pseudomonas aeruginosa in culture supernatants from clinical strains.
Louis D, Sorlier P, Wallach J
Clin Chem Lab Med 1998 May;36(5):295-8
PubMed ID: 9676386
|
Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.
Okuda K, Morihara K, Atsumi Y, Takeuchi H, Kawamoto S, Kawasaki H, Suzuki K, Fukushima J
Infect Immun 1990 Dec;58(12):4083-8
PubMed ID: 2123832
|
Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding.
Miyatake H, Hata Y, Fujii T, Hamada K, Morihara K, Katsube Y
J Biochem 1995 Sep;118(3):474-9
PubMed ID: 8690704
|
Pseudomonas aeruginosa alkaline protease: evidence for secretion genes and study of secretion mechanism.
Guzzo J, Pages JM, Duong F, Lazdunski A, Murgier M
J Bacteriol 1991 Sep;173(17):5290-7
PubMed ID: 1832151
|