Pseudomonas aeruginosa PAO1, PA5098 (hutH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5098
Name
hutH
Replicon chromosome
Genomic location 5739898 - 5741427 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253785.1
GI 15600291
Affymetrix PA5098_hutH_at
Entrez 878368
GenBank AAG08483.1
INSDC AAG08483.1
NCBI Locus Tag PA5098
protein_id(GenBank) gb|AAG08483.1|AE004922_8|gnl|PseudoCAP|PA5098
TIGR NTL03PA05099
UniParc UPI000012CE8F
UniProtKB Acc Q9HU85
UniProtKB ID HUTH_PSEAE
UniRef100 UniRef100_B7V3J1
UniRef50 UniRef50_P21310
UniRef90 UniRef90_Q02ER8

Product

Feature Type CDS
Coding Frame 1
Product Name
histidine ammonia-lyase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.55
Kyte-Doolittle Hydrophobicity Value 0.180
Molecular Weight (kDa) 53.8
Isoelectric Point (pI) 5.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 301 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004694 (505 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5098
Search term: hutH

Human Homologs

Ensembl 110, assembly GRCh38.p14
histidine ammonia-lyase [Source:HGNC Symbol;Acc:HGNC:4806]
E-value: 6.6e-108
Percent Identity: 44.3

References

Identification of serine-143 as the most likely precursor of dehydroalanine in the active site of histidine ammonia-lyase. A study of the overexpressed enzyme by site-directed mutagenesis.
Langer M, Reck G, Reed J, Rétey J
Biochemistry 1994 May 31;33(21):6462-7
PubMed ID: 8204579