Pseudomonas aeruginosa PAO1, PA1119 (yfiB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1119
Name
yfiB
Replicon chromosome
Genomic location 1212717 - 1213223 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249810.1
GI 15596316
Affymetrix PA1119_at
DNASU PaCD00006516
Entrez 881938
GenBank AAG04508.1
INSDC AAG04508.1
NCBI Locus Tag PA1119
protein_id(GenBank) gb|AAG04508.1|AE004542_4|gnl|PseudoCAP|PA1119
TIGR NTL03PA01120
UniParc UPI00000C525A
UniProtKB Acc Q9I4L6
UniProtKB ID Q9I4L6_PSEAE
UniRef100 UniRef100_W0YNR6
UniRef50 UniRef50_A6V969
UniRef90 UniRef90_A6V969

Product

Feature Type CDS
Coding Frame 1
Product Name
YfiB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.45
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 18.4
Isoelectric Point (pI) 8.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5EB0 MEMBRANE PROTEIN 10/17/15 crystal form II of YfiB belonging to P41 PSEUDOMONAS AERUGINOSA PAO1 2.8 X-RAY DIFFRACTION 100.0
6IKI SIGNALING PROTEIN 10/16/18 Crystal structure of YfiB(W55L) PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.204 X-RAY DIFFRACTION 99.4
5Y61 SIGNALING PROTEIN 08/10/17 YfiB-YfiR complexed with GMP PSEUDOMONAS AERUGINOSA 2.99 X-RAY DIFFRACTION 99.3
4ZHW SIGNALING PROTEIN 04/27/15 Crystal structure of a bacterial signalling protein (N-terminal truncation) PSEUDOMONAS AERUGINOSA PAO1 1.391 X-RAY DIFFRACTION 100.0
6IKJ SIGNALING PROTEIN 10/16/18 Crystal structure of YfiB(F48S) PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.76 X-RAY DIFFRACTION 99.4
4ZHY TRANSCRIPTION/SIGNALING PROTEIN 04/27/15 Crystal structure of a bacterial signalling complex PSEUDOMONAS AERUGINOSA PAO1 1.969 X-RAY DIFFRACTION 98.2
5EB1 MEMBRANE PROTEIN/TRANSCRIPTION 10/17/15 the YfiB-YfiR complex PSEUDOMONAS AERUGINOSA PAO1 1.8 X-RAY DIFFRACTION 99.3
4ZHV SIGNALING PROTEIN 04/27/15 Crystal structure of a bacterial signalling protein PSEUDOMONAS AERUGINOSA PAO1 1.585 X-RAY DIFFRACTION 100.0
6IKK SIGNALING PROTEIN 10/16/18 Crystal structure of YfiB(L43P) in complex with YfiR PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.19 X-RAY DIFFRACTION 99.4
5EAZ MEMBRANE PROTEIN 10/17/15 crystal form I of YfiB belonging to space groups P21 PSEUDOMONAS AERUGINOSA PAO1 2.151 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 407 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001079 (459 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1119
Search term: yfiB
Search term: YfiB

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
YfiBNR mediates cyclic di-GMP dependent small colony variant formation and persistence in Pseudomonas aeruginosa.
Malone JG, Jaeger T, Spangler C, Ritz D, Spang A, Arrieumerlou C, Kaever V, Landmann R, Jenal U
PLoS Pathog. 2010 Mar;6(3):e1000804
PubMed ID: 20300602