Pseudomonas aeruginosa PAO1, PA3053

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3053
Name
Replicon chromosome
Genomic location 3417113 - 3418120 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 7 transposon mutants in orthologs

Cross-References

RefSeq NP_251743.1
GI 15598249
Affymetrix PA3053_at
DNASU PaCD00007346
Entrez 882862
GenBank AAG06441.1
INSDC AAG06441.1
NCBI Locus Tag PA3053
protein_id(GenBank) gb|AAG06441.1|AE004730_5|gnl|PseudoCAP|PA3053
TIGR NTL03PA03053
UniParc UPI00000C58ED
UniProtKB Acc Q9HZF5
UniProtKB ID Q9HZF5_PSEAE
UniRef100 UniRef100_Q9HZF5
UniRef50 UniRef50_M5CPN2
UniRef90 UniRef90_Q9HZF5

Product

Feature Type CDS
Coding Frame 1
Product Name
probable hydrolytic enzyme
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 2.55
Kyte-Doolittle Hydrophobicity Value -0.234
Molecular Weight (kDa) 37.5
Isoelectric Point (pI) 7.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4F0J HYDROLASE 05/04/12 Crystal structure of a probable hydrolytic enzyme (PA3053) from Pseudomonas aeruginosa PAO1 at 1.50 A resolution Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 454 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000798 (2691 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3053

Human Homologs

Ensembl 110, assembly GRCh38.p14
epoxide hydrolase 2 [Source:HGNC Symbol;Acc:HGNC:3402]
E-value: 3.4e-12
Percent Identity: 34.1
Ensembl 110, assembly GRCh38.p14
epoxide hydrolase 2 [Source:HGNC Symbol;Acc:HGNC:3402]
E-value: 3.4e-12
Percent Identity: 34.1
Ensembl 110, assembly GRCh38.p14
epoxide hydrolase 2 [Source:HGNC Symbol;Acc:HGNC:3402]
E-value: 3.4e-12
Percent Identity: 34.1

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994