Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5493
|
Name |
polA
|
Replicon | chromosome |
Genomic location | 6183784 - 6186525 (- strand) |
Transposon Mutants | 5 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_254180.1 |
GI | 15600686 |
Affymetrix | PA5493_polA_at |
Entrez | 877794 |
GenBank | AAG08878.1 |
INSDC | AAG08878.1 |
NCBI Locus Tag | PA5493 |
protein_id(GenBank) | gb|AAG08878.1|AE004962_2|gnl|PseudoCAP|PA5493 |
TIGR | NTL03PA05494 |
UniParc | UPI00000C6035 |
UniProtKB Acc | Q9HT80 |
UniProtKB ID | DPO1_PSEAE |
UniRef100 | UniRef100_Q9HT80 |
UniRef50 | UniRef50_Q9HT80 |
UniRef90 | UniRef90_Q9HT80 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
DNA polymerase I
|
Synonyms | |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -32.14 |
Kyte-Doolittle Hydrophobicity Value | -0.135 |
Molecular Weight (kDa) | 99.8 |
Isoelectric Point (pI) | 4.75 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG005032 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5493
Search term: polA
Search term: DNA polymerase I
|
Ensembl
110, assembly
GRCh38.p14
|
DNA polymerase nu [Source:HGNC Symbol;Acc:HGNC:18870]
E-value:
7.2e-57
Percent Identity:
31.3
|
Ensembl
110, assembly
GRCh38.p14
|
DNA polymerase nu [Source:HGNC Symbol;Acc:HGNC:18870]
E-value:
7.2e-57
Percent Identity:
31.3
|
Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.
Joyce CM, Kelley WS, Grindley ND
J Biol Chem 1982 Feb 25;257(4):1958-64
PubMed ID: 6276402
|
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA
Nature 1985 Feb 28-Mar 6;313(6005):762-6
PubMed ID: 3883192
|
Structural principles for the inhibition of the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I by phosphorothioates.
Brautigam CA, Steitz TA
J Mol Biol 1998 Mar 27;277(2):363-77
PubMed ID: 9514742
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|